Recombinant cynomolgus monkey CSF-1-R protein (His tag) (ab220565)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
Description
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Product name
Recombinant cynomolgus monkey CSF-1-R protein (His tag)
See all CSF-1-R proteins and peptides -
Biological activity
Immobilized ab220565 at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His, Avitag (ab246167) with a linear range of 2-16 ng/mL.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Cynomolgus monkey -
Sequence
IPVIEPSGPELV VKPGETVTLR CVGNGSVEWD GPISPHWTLY SDGPSSVLTT NNATFQNTRT YRCTEPGDPL RGSAAIHLYV KDPARPWNVL AKEVVVFEDQ DALLPCLLTD PVLEAGVSLV RLRGRPLLRH TNYSFSPWHG FIIHRAKFIQ GQDYQCSALM GGRKVMSISI RLKVQKVIPG PPALTLVPEE LVRIRGEAAQ IVCSASNIDV DFDVFLQHNT TKLAIPQRSD FHDNRYQKVL TLSLGQVDFQ HAGNYSCVAS NVQGKHSTSM FFRVVESAYL DLSSEQNLIQ EVTVGEGLNL KVMVEAYPGL QGFNWTYLGP FSDHQPEPKL ANATTKDTYR HTFTLSLPRL KPSEAGRYSF LARNPGGWRA LTFELTLRYP PEVSVIWTSI NGSGTLLCAA SGYPQPNVTW LQCAGHTDRC DEAQVLQVWV DPHPEVLSQE PFQKVTVQSL LTTETLEHNQ TYECRAHNSV GSGSWAFIPI SAGARTHPPD EFLFTP -
Predicted molecular weight
57 kDa including tags -
Amino acids
99 to 596 -
Tags
His tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab220565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
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Form
Lyophilized -
Additional notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- C FMS
- CD 115
- CD115
see all -
Function
Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. -
Tissue specificity
Expressed in bone marrow and in differentiated blood mononuclear cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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Immobilized ab220565 at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His, Avitag (ab246167) with a linear range of 2-16 ng/mL.
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SDS-PAGE analysis of reduced ab220565 stained overnight with Coomassie Blue.
The protein migrates as 70-100 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab220565 has not yet been referenced specifically in any publications.