Recombinant Cynomolgus monkey B2M + FCGRT protein (Strep-tag) (ab191633)

Overview

Description

  • Nature

    Recombinant
  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Species

      Cynomolgus monkey
    • Tags

      His tag C-Terminus
    • Additional sequence information

      A heterodimer of Cynomolgus monkey FCGRT + B2M, (Q8SPV9 aa 24-297; Q6V7J5 aa 21-119) co-expressed by FCGRT plasmid and B2M plasmid, MW of 31.3 kDa (FCGRT), 13.1 kDa (B2M).

Specifications

Our Abpromise guarantee covers the use of ab191633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 95 % SDS-PAGE.

  • Form

    Lyophilised
  • Additional notes

    B2M is fused with Strep II-tag at the C-terminus and FCGRT is fused with his-tag at the C-terminus.

    This product is for the mature full length proteins. The signal peptides are not included.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.4
    Constituents: 95% PBS, 5% Trehalose

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.

General Info

  • Alternative names

    • beta chain of MHC class I molecules
    • beta-2-microglobin
    • Beta-2-microglobulin
    • c fragment of IgG, receptor, transporter, alpha
    • Fc Fragment of IgG, receptor transporter, alpha
    • FcRn
    • FcRn alpha chain
    • IgG Fc fragment receptor transporter alpha chain
    • IgG receptor FcRn large subunit p51
    • immunoglobulin receptor, intestinal, heavy chain
    • major histocompatibility complex class I-like Fc receptor
    • Neonatal Fc receptor
    • neonatal Fc-receptor for Ig
    see all
  • Relevance

    FCGRT + B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT has a possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Cellular localization

    FCGRT: Cell membrane; Single-pass type I membrane protein B2M: Secreted

Images

  • SDS-PAGE analysis of reduced ab191633 stained overnight with Coomassie Blue. DTT-reduced Protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

References

ab191633 has not yet been referenced specifically in any publications.

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