Bioactive grade

Recombinant cynomolgus monkey CD40 protein (Active) (ab220560)

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Functional Studies - Recombinant cynomolgus monkey CD40 protein (Active) (ab220560)
  • SDS-PAGE - Recombinant cynomolgus monkey CD40 protein (Active) (ab220560)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant cynomolgus monkey CD40 protein (Active)
    See all CD40 proteins and peptides

  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized ab220560 at 5 μg/mL (100 μL/well) can bind Mouse CD40, Fc Tag with a linear range of 1-6.6 ng/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    G7PG38

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Cynomolgus monkey

    • Sequence

      EPPTACREKQ YLINSQCCSL CQPGQKLVSD CTEFTETECL PCGESEFLDT WNRETRCHQH KYCDPNLGLR VQQKGTSETD TICTCEEGLH CTSESCESCV PHRSCLPGFG VKQIATGVSD TICEPCPVGF FSNVSSAFEK CRPWTSCETK DLVVQQAGTN KTDVVCGPQD RQR

    • Predicted molecular weight

      21 kDa including tags

    • Amino acids

      21 to 193

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab220560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

General Info

  • Alternative names

    • AI326936
    • B cell associated molecule CD40
    • B cell surface antigen CD40
    • B cell-associated molecule
    • B-cell surface antigen CD40
    • Bp50
    • CD 40
    • CD40
    • CD40 antigen
    • CD40 antigen (TNF receptor superfamily member 5)
    • CD40 molecule
    • CD40 molecule, TNF receptor superfamily member 5
    • CD40 protein
    • CD40 type II isoform
    • CD40L receptor
    • CDw40
    • GP39
    • HIGM1
    • IGM
    • IMD3
    • MGC9013
    • Nerve growth factor receptor related B lymphocyte activation molecule
    • OTTHUMP00000031699
    • OTTHUMP00000031700
    • p50
    • T-BAM
    • TBAM
    • TNF receptor superfamily member 5
    • TNFRSF5
    • TNR5_HUMAN
    • TRAP
    • Tumor necrosis factor receptor superfamily , member 5
    • Tumor necrosis factor receptor superfamily member 5
    • Tumor necrosis factor receptor superfamily member 5 precursor
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
    see all

  • Function

    Receptor for TNFSF5/CD40LG.

  • Tissue specificity

    B-cells and in primary carcinomas.

  • Involvement in disease

    Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

  • Sequence similarities

    Contains 4 TNFR-Cys repeats.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Images

  • Functional Studies - Recombinant cynomolgus monkey CD40 protein (Active) (ab220560)
    Functional Studies - Recombinant cynomolgus monkey CD40 protein (Active) (ab220560)

    Immobilized ab220560 at 5 μg/mL (100 μL/well) can bind Mouse CD40, Fc Tag with a linear range of 1-6.6 ng/mL.

  • SDS-PAGE - Recombinant cynomolgus monkey CD40 protein (Active) (ab220560)
    SDS-PAGE - Recombinant cynomolgus monkey CD40 protein (Active) (ab220560)

    SDS-PAGE analysis of reduced ab220560 stained overnight with Coomassie Blue.

    The protein migrates as 30-34 kDa on a SDS-PAGE gel under reducing conditions.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab220560? Please let us know so that we can cite the reference in this datasheet.

ab220560 has not yet been referenced specifically in any publications.

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