Bioactive grade

Recombinant cynomolgus monkey LAG-3 protein (Fc Chimera Active) (ab220581)

Description

  • Product name

    Recombinant cynomolgus monkey LAG-3 protein (Fc Chimera Active)
    See all LAG-3 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized ab220581 at 2 μg/mL (100 μL/well) can bind Anti-LAG3 MAb, Human IgG1 with a linear range of 1-51 ng/mL.

  • Purity

    > 80 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Cynomolgus monkey
    • Sequence

      APVKPPQPGAEIS VVWAQEGAPA QLPCSPTIPL QDLSLLRRAG VTWQHQPDSG PPAXAPGHPP VPGHRPAAPY SWGPRPRRYT VLSVGPGGLR SGRLPLQPRV QLDERGRQRG DFSLWLRPAR RADAGEYRAT VHLRDRALSC RLRLRVGQAS MTASPPGSLR TSDWVILNCS FSRPDRPASV HWFRSRGQGR VPVQGSPHHH LAESFLFLPH VGPMDSGLWG CILTYRDGFN VSIMYNLTVL GLEPATPLTV YAGAGSRVEL PCRLPPAVGT QSFLTAKWAP PGGGPDLLVA GDNGDFTLRL EDVSQAQAGT YICHIRLQGQ QLNATVTLAI ITVTPKSFGS PGSLGKLLCE VTPASGQEHF VWSPLNTPSQ RSFSGPWLEA QEAQLLSQPW QCQLHQGERL LGAAVYFTEL SSPGAQRSGR APGALRAGH
    • Predicted molecular weight

      73 kDa including tags
    • Amino acids

      18 to 449
    • Additional sequence information

      Fused with a human IgG1 Fc tag (Pro 100 - Lys 330; P01857) at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab220581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Additional notes

    This product was previously labelled as Lymphocyte Activation Gene 3.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.44% L-Arginine, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD223
    • CD223 antigen
    • FDC protein
    • LAG-3
    • Lag3
    • LAG3_HUMAN
    • Lymphocyte activating 3
    • Lymphocyte activation gene 3
    • Lymphocyte activation gene 3 protein
    • Protein FDC
    see all
  • Function

    Involved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificity

    On cell surface of activated NK and T-lymphocytes.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Cynomolgus LAG-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
  • Flow Cytometry assay shows that�Cynomolgus LAG-3, Fc�Tag can bind to daudi cell surface. The concentration of LAG-3 used is 1 µg/ml.
  • Immobilized Cynomolgus LAG-3, Fc Tag at 2 µg/mL (100 µL/well) can bind Anti-Human LAG3 MAb, Human IgG1 with a linear range of 8-31 ng/mL.
  • SDS-PAGE analysis of reduced ab220581 stained overnight with Coomassie Blue.

    The protein migrates as 90-100 kDa under reducing conditions (SDS-PAGE) due to glycosylation.

References

ab220581 has not yet been referenced specifically in any publications.

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