Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, ELISA
Description
-
Product name
Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active)
See all TIGIT proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque CD155, His Tag at 5 μg/mL (100 μL/well) can bind ab223105 with a linear range of 0.02-0.313 μg/mL.
-
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Cynomolgus monkey -
Predicted molecular weight
40 kDa including tags -
Amino acids
89 to 208 -
Tags
Fc tag C-Terminus -
Additional sequence information
Fused with human IgG1 Fc tag at the C-terminus. In the region Met 89 - Ile 208, the AA sequence of Cynomolgus monkey and Rhesus macaque TIGIT are homologus.
-
Specifications
Our Abpromise guarantee covers the use of ab223105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Functional Studies
ELISA
-
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
-
Alternative names
- DKFZp667A205
- ENSMUSG00000071552
- FLJ39873
see all -
Function
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. -
Tissue specificity
Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level). -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Cell membrane. - Information by UniProt
Images
-
Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)
Immobilized Rhesus macaque CD155, His Tag at 5 μg/mL (100 μL/well) can bind ab223105 with a linear range of 0.02-0.313 μg/mL.
-
SDS-PAGE - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)ab223105 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The protein migrates as 45-55 kDa due to glycosylation.
-
Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)Loaded ab223105 on Protein A Biosensor, can bind Human CD155, His Tag with an affinity constant of 8.2 μM as determined in BLI assay.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab223105 has not yet been referenced specifically in any publications.
