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Immunology Adaptive Immunity T Cells Non-CD
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Bioactive grade

Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)

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Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)
  • SDS-PAGE - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)
  • Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: Fc tag C-Terminus
  • Suitable for: SDS-PAGE, Functional Studies, ELISA

Description

  • Product name

    Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active)
    See all TIGIT proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque CD155, His Tag at 5 μg/mL (100 μL/well) can bind ab223105 with a linear range of 0.02-0.313 μg/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

    G7NXM4
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Cynomolgus monkey
    • Predicted molecular weight

      40 kDa including tags
    • Amino acids

      89 to 208
    • Tags

      Fc tag C-Terminus
    • Additional sequence information

      Fused with human IgG1 Fc tag at the C-terminus. In the region Met 89 - Ile 208, the AA sequence of Cynomolgus monkey and Rhesus macaque TIGIT are homologus.

Specifications

Our Abpromise guarantee covers the use of ab223105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    ELISA

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris

    Lyophilized from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

General Info

  • Alternative names

    • DKFZp667A205
    • ENSMUSG00000071552
    • FLJ39873
    • RGD1563191
    • T cell immunoreceptor with Ig and ITIM domains
    • TIGIT
    • TIGIT_HUMAN
    • V-set and immunoglobulin domain containing 9
    • V-set and immunoglobulin domain-containing protein 9
    • V-set and transmembrane domain containing 3
    • V-set and transmembrane domain-containing protein 3
    • VSIG9
    • VSTM3
    • Washington University cell adhesion molecule
    • WUCAM
    see all
  • Function

    Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
  • Tissue specificity

    Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level).
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain

    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q495A1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)
    Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)

    Immobilized Rhesus macaque CD155, His Tag at 5 μg/mL (100 μL/well) can bind ab223105 with a linear range of 0.02-0.313 μg/mL.

  • SDS-PAGE - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)
    SDS-PAGE - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)

    ab223105 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The protein migrates as 45-55 kDa due to glycosylation.

  • Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)
    Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)

    Loaded ab223105 on Protein A Biosensor, can bind Human CD155, His Tag with an affinity constant of 8.2 μM as determined in BLI assay.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab223105? Please let us know so that we can cite the reference in this datasheet.

    ab223105 has not yet been referenced specifically in any publications.

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