Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)
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Description
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Product name
Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active)
See all TIGIT proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human CD155, His Tag at 10 μg/ml (100 μl/well) can bind ab223105 with a linear range of 2.22 - 20 μg/ml.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Cynomolgus monkey -
Predicted molecular weight
40 kDa including tags -
Amino acids
89 to 209 -
Additional sequence information
Fused with human IgG1 Fc tag at the C-terminus.
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Specifications
Our Abpromise guarantee covers the use of ab223105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilised -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
General Info
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Alternative names
- DKFZp667A205
- ENSMUSG00000071552
- FLJ39873
see all -
Function
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. -
Tissue specificity
Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level). -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)Cynomolgus / Rhesus macaque TIGIT (Fc Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. -
SDS-PAGE - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)SDS-PAGE analysis of ab223105, stained overnight with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
As a result of glycosylation, the protein migrates as 40-50 kDa under reducing conditions, and 80-116 kDa under non-reducing conditions.
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Functional Studies - Recombinant cynomolgus monkey TIGIT protein (Fc Chimera Active) (ab223105)Immobilized human CD155, His Tag at 10 μg/ml (100 μl/well) can bind ab223105 with a linear range of 2.22 - 20 μg/ml.
Datasheets and documents
References
ab223105 has not yet been referenced specifically in any publications.
