Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Additional sequence information
      The protein contains the Cytomegalovirus (CMV) gB immunodominant regions.

Specifications

Our Abpromise guarantee covers the use of ab43040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Immunoreactive with sera of CMV infected individuals.
  • Applications

    ELISA

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Sepharose derived purification.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.20
    Constituents: 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol

General Info

  • Alternative names
    • CMV glycoprotein B
    • Glycoprotein B
    • HCMV
    • HHV5 glycoprotein B
    • UL55
    see all
  • Relevance
    Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
  • Cellular localization
    Virus envelope.

References

This product has been referenced in:
  • Pires F  et al. A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification. J Pharm Biomed Anal 156:372-378 (2018). Read more (PubMed: 29758495) »
  • Crawford LB  et al. Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice. Sci Rep 7:937 (2017). ELISA . Read more (PubMed: 28428537) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your enquiry. This product was made from a 57 amino acid long sequence and a GST fusion. The total molecular weight should be 32.2kDA. Therefore, please rest assured that the band you are seeing is indeed the Cytomegalovirus Glycoprotein B protein. I am afraid that there has been an error when the datasheet was created and the information has now been removed from the datasheet. We sincerely apologize for any inconvenience caused.

Read More

Answer



This protein (ab43040: Active Cytomegalovirus Glycoprotein B protein fragment) was developed for research purposes for ELISA and Western blot applications only - but not for diagnostic or internal use in vivo. Therefore, no endotoxin tests were conducted.

We used conserved sequences from CMV strains AD169 and Towne to construct ab43040.

Read More

Answer

Thank you for your inquiry.

I can confirm that the immunogen for ab43040 is from within the amino acid range 11-67. I am sorry the exact sequence is proprietary.

Unfortunately, we are not able to release the immunogen sequence forab54103as it is proprietary information. We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

I hope this will still be helpful. Should you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for supplying that information.


I have been in contact with the lab and they suggested that maybe the PAGE sample was heated too long (5 minutes) prior to loading. It is most likely that a specific smallmolecular weight amino acidpeptide was cleaved off. The short peptide that was cleaved is too small to stay on the PAGE and to be detected later on by the western blot.

If there is anything else I can help you with, please let me know.

Read More

Answer

Thank you for contacting Abcam.


I have been in contact with the lab about your enquiry as to whether a doublet was expected on a western blot, when you ran ab43040 on a western blot. They have asked for some more information:



1 .How much protein was on the PAGE?

2. Was the protein pre-heated in loading buffer prior to loading?

3. Can you supply an image of the western blot?



I look forward to your reply and helping resolve this issue.

Read More

Answer

Sorry for the late reply. The protein contains the CMV gB immunodominant regions without the signal peptide. I hope this is helpful. Please contact us again if you have any further questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up