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I was wondering if it was normal that I detect two bands when I perform a Western Blot against the gB protein with a CMV anti-gB monoclonal antibody (2F12 clone). It looks like a doublet on the gel, around 37 kDa of MW. -4ug loaded -CMV gB mouse monoclonal ab 1:1000 -goat anti-mouse ab 1:50,000 -Detection WestFemto kit, 10 min exposure
Asked on May 22 2012
Thank you for supplying that information.
I have been in contact with the lab and they suggested that maybe the PAGE sample was heated too long (5 minutes) prior to loading. It is most likely that a specific smallmolecular weight amino acidpeptide was cleaved off. The short peptide that was cleaved is too small to stay on the PAGE and to be detected later on by the western blot.
If there is anything else I can help you with, please let me know.
Answered on May 22 2012