Key features and details
- Expression system: Escherichia coli
- Tags: GST tag N-Terminus
- Suitable for: WB, ELISA
Product nameRecombinant Cytomegalovirus pp65 protein (297-510aa)
See all Cytomegalovirus pp65 proteins and peptides
Expression systemEscherichia coli
Protein lengthProtein fragment
Amino acids297 to 510
TagsGST tag N-Terminus
DescriptionRecombinant Cytomegalovirus pp65 protein
Our Abpromise guarantee covers the use of ab54103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol (glycerin, glycerine)
- 65 kDa matrix phosphoprotein
- 65 kDa phosphoprotein
- Tegument protein pp65
- Tegument protein UL83
RelevanceCytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab54103 has not yet been referenced specifically in any publications.