Recombinant Cytomegalovirus pp65 protein (297-510aa) (ab54103)

Submit a review Q&A (2)

Key features and details

  • Expression system: Escherichia coli
  • Tags: GST tag N-Terminus
  • Suitable for: WB, ELISA

Description

  • Product name

    Recombinant Cytomegalovirus pp65 protein (297-510aa)
    See all Cytomegalovirus pp65 proteins and peptides

  • Expression system

    Escherichia coli

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Amino acids

      297 to 510

    • Tags

      GST tag N-Terminus

  • Description

    Recombinant Cytomegalovirus pp65 protein

Specifications

Our Abpromise guarantee covers the use of ab54103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • 65 kDa matrix phosphoprotein
    • 65 kDa phosphoprotein
    • Tegument protein pp65
    • Tegument protein UL83

  • Relevance

    Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab54103? Please let us know so that we can cite the reference in this datasheet.

ab54103 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

One of our customers need to develop Sandwich ELISA for below


1- ab54103

So they will need below for this CMV proteins

2- and two antibodies compatible to this antigen, one of them conjugated to HRP

Read More
Answer

Unfortunately, we only have 5 Anti-Cytomegalovirus pp65 antibodies only two of which have been tested in ELISA. Unfortunately these two antibodies have not been validated for use in sELISA together and therefore we cannot guarantee that they will work. In addition, we do not have an Anti-Cytomegalovirus pp65 antibody conjugated to HRP.

I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

Read More

Question

Hello,

I am following up from a phone call that I had last week. I requested information on the protein sequence of the following peptides:

Cytomegalovirus pp65 IE protein (ab54103)

Cytomegalovirus Glycoprotein B protein (ab43040)

Would you be able to tell me the amino acid sequence and what position (ie. what number AA it starts and ends at)?

I am trying to find monoclonal antibodies that are specific to these two proteins – would you be able to recommend any that you make?

It would be greatly appreciated if you could get back to me as soon as possible as it has been a number of days since the phone call.

The person I spoke to on the phone was Kate.

Many thanks

Read More
Answer

Thank you for your inquiry.

I can confirm that the immunogen for ab43040 is from within the amino acid range 11-67. I am sorry the exact sequence is proprietary.

Unfortunately, we are not able to release the immunogen sequence forab54103as it is proprietary information. We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

I hope this will still be helpful. Should you have any further questions, please do not hesitate to contact us.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com