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I would like to detect Dengue NS1 virus using microfluidics platform. I am looking for Dengue NS1 capture antibodies, antigens and detection antibodies. I looked in to some of the items listed from your website. They are:
ab41490, ab41616, ab41623, ab64456,ab102884,ab138696, ab1206.
I need to detect this Dengue NS1 virus as sandwich immunoassay. What type of negative and positive control I need to use for accurate detection of Dengue. Do you have any Dengue NS1 Elisa kits? It will be great if you can provide an overview of dengue detection how the Dengue IgG and IgM detection is different from the NS1 detection.
Can you please suggest me what are the things I need to purchase for my experiments.
Asked on Nov 06 2012
Thank you for your interest in our products.
The two kits we have for detection of Dengue virus-specific IgG or IgM ab108728 and ab108729, are for detection of antibodies in serological samples that detect Dengue virus. So, they detect an immune response to the virus, rather than the virus itself. On the other hand, antibodies that are raised against the virus, such as antibody ab138696, bind to the virus itself.
For a positive control, I recommend the recombinant NS1 protein, ab64456. A negative control would simply be a blank control, buffer with no protein.
Most of the antibodies you selected have not, to our knowledge, been tested in ELISA of any kind. For sandwich ELISA, I am hoping we can add the detection antibody that complements the capture antibody, ab138696. I expect we will have this on our website in just a few days. I will e-mail you when we do add it.
Like ab138696, it is a mouse monoclonal antibody raised against the NS1 protein. We do not have either antibody in a conjugated format, but we do have conjugation products that make conjugating an antibody simple, which may be necessary, depending on your platform. Enter "Easylink" in the search box on our website to see our selection of conjugation kits. Before conjugation, the antibody will need to be concentrated to the appropriate concentration, at least 1 mg/ml. The product for concentrating an antibody is ab102776, or ab102778 for three units.
https://www.abcam.com/index.html?datasheet=102776 (or use the following: https://www.abcam.com/index.html?datasheet=102776).
I will contact you when we have the partner for ab138696 up on the website. Please let me know if you have any questions in the meantime.
Answered on Nov 06 2012