Description

  • Product name

    Recombinant Dengue Virus 4 NS1 glycoprotein
  • Purity

    > 95 % SDS-PAGE.
    ab181957 is 0.2µm filter sterilised.
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Amino acids

      776 to 1127
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Sequence strain: Dominica/814669/1981

Specifications

Our Abpromise guarantee covers the use of ab181957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.50
    Constituents: Tris, 0.87% Sodium chloride

General Info

  • Relevance

    Dengue virus type 4 (strain Dominica/814669/1981) (DENV-4). Non-structural protein 1 is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome.

References

ab181957 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The DENV NS1 protein products are all full length mammalian recombinants produced in a human cell line. They are hexameric in conformation as demonstrated by a single sharp peak at around 440kDa via SEC. They are correctly glycosylated due to the fact they are produced in human cells a band shift is seen when treated with endoglycosidases. The serotypes we used are as follows:



A leading Dengue research lab has confirmed they are reactive to dengue anti-sera (whereas NS1 produced in bacterial cells is not reactive).

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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