Recombinant DNA ligase protein (His tag) (ab238367)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant DNA ligase protein (His tag)
See all DNA ligase proteins and peptides -
Purity
> 85 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Sequence
RPQTSRLGRVNPVAEVEPTRLSGALIQRVTAHHYAMVRDRGIGPGARIRM SRSGEVIPKIEEVVKPVAAAQLDIPAHCPSCGSDLAWDGDYLFCLNNMSC PAQITNSMEHFFKILGNVDGFGPSSVQRLYEGGLVDLPALYAMESADFEQ VGFGPKQATNMVEQLNRSREELIEDWRFLAAFGVHRLGMGNCEKLLRYVR LEDVFNLSEEQIVAEIKGFQEKTAHSICAGLAGIKDLFFQLFALGFKLEP TPLLAEGGQGEKSPISGKTIVFTGAMLAASRGDMEKQAKALGATVGKSIT GKTDMLVTGQRVGATKMAKAETLGIAILSEEAYRQLVSLD -
Predicted molecular weight
40 kDa including tags -
Amino acids
340 to 679 -
Tags
His tag N-Terminus -
Additional sequence information
Desulfotalea psychrophila (strain LSv54 / DSM 12343). N-terminal 10xHis-tagged.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab238367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- DNA ligase NAD(+) dependent
- ligA
- Polydeoxyribonucleotide synthase [NAD(+)]
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Relevance
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab238367 has not yet been referenced specifically in any publications.