Recombinant DNA protection during starvation protein (ab203523)

Description

  • Product name

    Recombinant DNA protection during starvation protein
    See all DNA protection during starvation protein proteins and peptides
  • Purity

    > 95 % Densitometry.

  • Endotoxin level

    < 1.000 Eu/µl
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Helicobacter pylori
    • Sequence

      MKHHHHHHAS MKTFEILKHL QADAIVLFMK VHNFHWNVKG TDFFNVHKAT EEIYEEFADM FDDLAERIVQ LGHHPLVTLS EAIKLTRVKE ETKTSFHSKD IFKEILEDYK YLEKEFKELS NTAEKEGDKV TVTYADDQLA KLQKSIWMLQ AHLA
    • Predicted molecular weight

      18 kDa
    • Amino acids

      1 to 144
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab203523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    LC-MS/MS
  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.50
    Constituents: 99% Tris buffer, 0.29% Sodium chloride

    Lyophilized from a 0.4 µM filtered solution.

  • Reconstitution
    Add 200 µL of deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

General Info

  • Alternative names

    • Bacterioferritin
    • dps
    • HP-NAP
    • NAP A
    • napA
    • Neutrophil-activating protein A
    see all
  • Relevance

    Function: Protects DNA from oxidative damage by sequestering intracellular Fe2+ ion and storing it in the form of Fe3+ oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe2+ ions, which prevents hydroxyl radical production by the Fenton reaction (By similarity). Required for the survival in the presence of oxidative stress. Dps is also a virulence factor that activates neutrophils, mast cells and monocytes. It binds to neutrophil-glycosphingolipids and to sulfated carbohydrates on mucin. It might have a role in the accumulation of neutrophils and monocytes at the site of infection. Induces superoxide anion generation, adhesion and chemotaxis of neutrophils, through a pertussis toxin-sensitive pathway involving MAP kinases.

Images

  • 14% SDS-PAGE analysis of ab203523. 

    Lane 1: Reduced and boiled sample, 2.5 μg/lane
    Lane 2: Non-reduced and non-boiled sample, 2.5 μg/lane

References

ab203523 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203523.
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