Product nameRecombinant Dog Creatine Kinase MM protein
See all Creatine Kinase MM proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
SequenceHHHHHHGSGGSGPFGNTHNKFKLNYKPEEEYPDLTKHNNHMAKALTPEIY KKLRDKETPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEESYQVFKDLF DPIIQDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKG YTLPPHCSRGERRAVEKLSIEALNSLTGEFKGKYYPLKSMTEQEQQQLID DHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVIS MQKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNEHLGYVLTCPSNLGT GLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDISNA DRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
Molecular weight44 kDa including tags
Amino acids2 to 381
TagsHis tag N-Terminus
Additional sequence informationThis product is the mature full length protein from aa 2 to 381. The initiator methionine is not included.
Our Abpromise guarantee covers the use of ab197789 in the following tested applications.
Purity> 90 % SDS-PAGE.
Product was previously marketed under the MitoSciences sub-brand.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.03% EDTA, 10% Trehalose, 89% PBS
- Creatine kinase M
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain.
- Information by UniProt
Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1 µg/ml +
Recombinant Dog Creatine Kinase MM protein (ab197789) at 0.1 µg
HRP conjugated Goat anti-mouse IgG (H&L) at 1/5000 dilution
Developed using the ECL technique.
Exposure time: 5 minutes
Samples run on a 4-20% acrylamide gel, performed under reducing conditions and developed using the ECL technique.
Membrane was blocked with 5% Milk in PBS, Primary antibody was incubated in 1% Milk in PBS and secondary antibody was incubated in 1X Blocking buffer (ab126587).
Predicted CKM band size: 43.1 kDa
Observed CKM band size: 43 kDa
Example of canine CKM protein performance when ran in an ELISA (Canine CKM SimpleStep ELISA, ab197749).
ab197789 has not yet been referenced specifically in any publications.