Description

  • Product name

    Recombinant dog IL-2 protein (Active)
    See all IL-2 proteins and peptides
  • Biological activity

    CTLL-2 cell proliferation: ED50 ≤ 5 ng/mL (≥ 2.0 x 105 units/mg)

  • Purity

    > 95 % SDS-PAGE.
    Produced using animal-free processes and contains only animal-free raw materials.
  • Endotoxin level

    <=1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Dog
    • Sequence

      MAPITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPK KATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLK GSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT
    • Predicted molecular weight

      16 kDa
    • Amino acids

      21 to 155
    • Additional sequence information

      Full length mature chain without signal peptide. Genetically modified E.coli.

Specifications

Our Abpromise guarantee covers the use of ab233688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Storage:

    12 months from date of receipt when stored at -20°C to -80°C as supplied. 

    1 month when stored at 4°C after reconstituting as directed.

    3 months when stored at -20°C to -80°C after reconstituting as directed.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at -20°C or -80°C.

    pH: 8.50
    Constituent: 0.08% Sodium bicarbonate

    Lyophilized from a sterile (0.2 micron) filtered aqueous solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile 10 mM sodium bicarbonate, pH 8.5 at 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

General Info

  • Alternative names

    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • OTTHUMP00000164090
    • POIL2
    • T Cell Growth Factor
    • T-cell growth factor
    • TCGF
    see all
  • Function

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in disease

    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similarities

    Belongs to the IL-2 family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Recombinant dog IL2 induced proliferation of CTLL-2 cells.

  • SDS-PAGE - Recombinant dog IL2 protein (Active) (ab233688) at 1 µg.
    Lane 1: Non-reducing conditions.
    Lane 2: Reducing conditions.
    4-20% Tris-Glycine gel stained with Coomassie Blue.
    Expected MW 15.6 kDa.

     

References

ab233688 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233688.
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