Recombinant Dog Interferon alpha 1 protein (ab169720)

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Key features and details

  • Expression system: Yeast
  • Purity: > 95% Ion Exchange Chromatography
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Dog Interferon alpha 1 protein
    See all Interferon alpha 1 proteins and peptides

  • Purity

    > 95 % Ion Exchange Chromatography.
    Purity: >95% as visualized by SDS-PAGE analysis.

  • Expression system

    Yeast

  • Accession

    P81255

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Dog

    • Sequence

      CHLPDTHGLR NWRVLTLLGQ MRRLSAGSCD HYTNDFAFPK ELFDGQRLQE AQALSVVHVM TQKVFHLFCP DTSSAPWNMT LLEELCSGLS EQLDDLEACP LQEAGLAETP LMHEDSTLRT YFQRISLYLQ DRNHSPCAWE MVRAEIGRSF FSSTILQERI RRRK

    • Predicted molecular weight

      19 kDa

    • Amino acids

      24 to 187

    • Additional sequence information

      Mature protein without signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab169720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    Constituents: 90% PBS, 10% Trehalose

  • Reconstitution
    Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. Store in working aliquots at -20°C. Avoid repeated freeze/thaw cycles.

General Info

  • Alternative names

    • IFL
    • IFN
    • IFN alpha
    • IFN alpha 1/13
    • IFN alpha 1b
    • IFN alphaD
    • IFN-alpha-1/13
    • IFNA@
    • IFNA1
    • IFNA1_HUMAN
    • IFNA13
    • Interferon alpha 1
    • Interferon alpha-1/13
    • Interferon alpha-D
    • Interferon alpha1
    • Interferon, alpha 13
    • LeIF D
    see all

  • Function

    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

  • Sequence similarities

    Belongs to the alpha/beta interferon family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab169720? Please let us know so that we can cite the reference in this datasheet.

ab169720 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Canine cell line stimulation and induction of ISG expression

 Excellent
Abreviews
abreview image
Application
Functional Studies
The product was dissolved in 0.1% BSA-PBS and added to a canine cell line at a final concentration of 0.05 ug/mL in the culture media. RNA was extracted from the cells at different time points post IFN stimulation and converted to cDNA. We ran qPCR to look at a canine ISG expression, which reached peak induction at the 24 hr time point.

Abcam user community

Verified customer

Submitted Sep 24 2018

canine cell line stimulation

 Excellent
Abreviews
abreview image
Application
Cellular Activation
Using this recombinant protein, we tried to stimulate canine epithelial cell.
Ab169720 was dissolved in 0.1% BSA-PBS at 250ul.
It was added to the culture medium.
The final concentration was 100ug/ml.
12 hours post stimulation, the cells were infected with some virus.
24 hours post infection, the cells were fixed and the viral protein was detected by immunofluorescence method.
The number and amount of detected viral protein were fewer in stimulated cells than negative control.

Abcam user community

Verified customer

Submitted Jan 29 2014

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