Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 50% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant dog PDE4B protein
See all PDE4B proteins and peptides
Specific activity: ≥ 3882 pmol/min/µg
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198483 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Purity>= 50 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDH LVPRGSGSGIQKKSRSVMAVTADDN VKDYLE CSLSKSYSSSSNTLGIDLWRGRRCCSGNLQLPPLAQRQSERARTPEGDGI SRPTTLPLMTLPSIAITTVSQECFDVENGPSPGRSPLDPQASSSSGLVLH ATFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSLPSEQHGDDLIVTPFAQ VLASLRSVRNNFTVLTNLHGASNKRSPAATSQPPVSRVNLQEESYQKLAM ETLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQ VSEYISNTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSL NNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYA IFQERDLLKTFKISSDTFVTYMMTLEDHYHSDVAYHNSLHAADVAQSTHV LLSTPALDAVFTDLEILATIFAAAIHDVDHPGVSNQFLINTNSELALMYN DESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQTLRKMVIDMVLATDMS KHMSLLADLKTMVETKKVTSSGVLL LDNYTDRIQVLRNMVHCADLSNP TKSLELYRQWTDRIMEEFFQQGDKERER GMEISPMCDKHTASVEKSQV GFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPPLDE QNRDCQGLMEKFQFELTLEEEDSEGPDKEGEGHNYFSSTKTLCVIDPEHR DSLGDTDGDTGAENKTPID
Predicted molecular weight110 kDa including tags
Amino acids2 to 737
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab198483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic phosphodiesterase 4B
- cAMP-specific 3''
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
Tissue specificityExpressed in brain, heart, lung and skeletal muscle.
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198483 has not yet been referenced specifically in any publications.