Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant dog PDE4D protein
See all PDE4B proteins and peptides
Specific Activity: ≥ 5665 pmol/min/µg
Unit Definition: One unit is defined as the amount of ab198425 that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198425 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Purity> 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHLVPRGSGSGIQSIIMKPRSRSTSSLRTTEAACFDV DNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKS MSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRS PMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMAS NKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKE KKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWG LHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHY HADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVD HPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLT KKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNY SDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGME ISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNR EWYQSTIPQSPSPAPDEQEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQ VEEDTSCSDSKTLCTQDSESTEIPLDEQVEEETVGEEENSQPEACVIEDH SPDT
Predicted molecular weight104 kDa including tags
Amino acids2 to 679
TagsGST tag N-Terminus
DescriptionRecombinant dog PDE4B protein
Our Abpromise guarantee covers the use of ab198425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 20% Glycerol (glycerin, glycerine), 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic phosphodiesterase 4B
- cAMP-specific 3''
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
Tissue specificityExpressed in brain, heart, lung and skeletal muscle.
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198425 has not yet been referenced specifically in any publications.