Description

  • Product name

    Recombinant dog SCF protein
    See all SCF proteins and peptides
  • Biological activity

    The biological activity of recombinant canine SCF was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 1-2 ng/ml.

  • Purity

    > 95 % Ion Exchange Chromatography.

  • Expression system

    Yeast
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Dog
    • Sequence

      GICGKRVTDDVKDVTKLVANLPKDYKIALKYVPGMDVLPSHCWISVMVEQ LSVSLTDLLDKFSNISEGLSNYSIIDKLVKIVDDLVECTEGYSFENVKKA PKSPELRLFTPEEFFRIFNRSIDAFKDLETVASKSSECVVSSTLSPDKDS RVSVTKPFMLPPVAASSLRNDSSSSNRKASNSIGDSNLQWAAMALPAFFS LVIGFAFGALYWKKKQPNLTRTVENIQINEEDNEISMLQEKEREFQEV
    • Predicted molecular weight

      28 kDa
    • Amino acids

      27 to 274

Specifications

Our Abpromise guarantee covers the use of ab222170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C long term. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Stable for 12 months at -20°C. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..

    Constituents: PBS, 10% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.

General Info

  • Alternative names

    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • Function

    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • Involvement in disease

    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • Sequence similarities

    Belongs to the SCF family.
  • Developmental stage

    Acts in the early stages of hematopoiesis.
  • Post-translational
    modifications

    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • Cellular localization

    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt

References

ab222170 has not yet been referenced specifically in any publications.

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