Description

  • Product name

    Recombinant dUTPase protein
    See all dUTPase proteins and peptides
  • Biological activity

    Specific Activity: 1MIU/mg. Unit Defenition: One unit of enzyme catalyzes hydrolysis of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
  • Purity

    > 95 % SDS-PAGE.
    Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Pyrococcus furiosus

Specifications

Our Abpromise guarantee covers the use of ab68279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    The enzymes of Pyrococcus furiosus are extremely thermostable. Consequently the DNA Polymerase from Pyrococcus furiosus (also known as Pfu DNA polymerase) can be used in the polymerase chain reaction (PCR) DNA amplification process. It hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing contaminating dUTP levels and providing the dUMP for dTTP biosynthesis.

    Enhancing PCR amplication: 50µl of Pfu PCR reaction system with 1-3u of dUTPase to amplify genomic DNA target up to 15-19 kb in length.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.20
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.1% Tween, 0.00292% EDTA, 50% Glycerol, 0.1% Igepal

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform
    • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
    • Deoxyuridine triphosphatase
    • dut
    • DUT_HUMAN
    • dUTP nucleotidohydrolase
    • dUTP pyrophosphatase
    • dUTPase
    • FLJ20622
    see all
  • Function

    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
  • Tissue specificity

    Found in a variety of tissues.
  • Pathway

    Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
  • Sequence similarities

    Belongs to the dUTPase family.
  • Post-translational
    modifications

    Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
  • Cellular localization

    Mitochondrion and Nucleus.
  • Information by UniProt

References

ab68279 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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