Recombinant E. coli Acetate Kinase protein (ab187412)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, MS

Description

  • Product name

    Recombinant E. coli Acetate Kinase protein
    See all Acetate Kinase proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Escherichia coli

    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA

    • Predicted molecular weight

      46 kDa including tags

    • Amino acids

      1 to 400

    • Tags

      His tag N-Terminus

    • Additional sequence information

      NP_416799.1.

Specifications

Our Abpromise guarantee covers the use of ab187412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.87% Sodium chloride, 20% Glycerol (glycerin, glycerine), 0.02% DTT, 0.32% Tris HCl

General Info

  • Alternative names

    • acetate kinase A and propionate kinase 2
    • Acetokinase
    • ack
    • EC=2.7.2.1

  • Relevance

    Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically.

  • Cellular localization

    Cytoplasmic

Images

  • SDS-PAGE - Recombinant <em>E. coli </em> Acetate Kinase protein (ab187412)
    SDS-PAGE - Recombinant E. coli Acetate Kinase protein (ab187412)

    15% SDS-PAGE analysis of ab187412 (3μg).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab187412? Please let us know so that we can cite the reference in this datasheet.

ab187412 has not yet been referenced specifically in any publications.

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