Recombinant E. coli Acetate Kinase protein (ab187412)

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SDS-PAGE - Recombinant <em>E. coli </em> Acetate Kinase protein (ab187412)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant E. coli Acetate Kinase protein
      See all Acetate Kinase proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Escherichia coli

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA

      • Predicted molecular weight

        46 kDa including tags

      • Amino acids

        1 to 400

      • Tags

        His tag N-Terminus

      • Additional sequence information

        NP_416799.1.

    Specifications

    Our Abpromise guarantee covers the use of ab187412 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.87% Sodium chloride, 20% Glycerol (glycerin, glycerine), 0.02% DTT, 0.32% Tris HCl

    General Info

    • Alternative names

      • acetate kinase A and propionate kinase 2
      • Acetokinase
      • ack
      • EC=2.7.2.1

    • Relevance

      Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically.

    • Cellular localization

      Cytoplasmic

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> Acetate Kinase protein (ab187412)
      SDS-PAGE - Recombinant E. coli Acetate Kinase protein (ab187412)

      15% SDS-PAGE analysis of ab187412 (3μg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab187412? Please let us know so that we can cite the reference in this datasheet.

    ab187412 has not yet been referenced specifically in any publications.

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