Key features and details
- Expression system: Escherichia coli
- Purity: >= 78% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Conjugation, Purification, SDS-PAGE
Product nameRecombinant E. coli BirA protein
See all BirA proteins and peptides
BirA (16 µg/mL) completely biotinlabels 10 µM of Avi-tag substrate at 30°C for 30 minutes in reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM dBiotin.
Purity>= 78 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLVPRGSKDNTVPLKLIALLANGEFHSGEQL GETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQ LDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFS PFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPN DLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWIT LQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRP VKLIIGDKEIFGISRGIDKQGALLLE QDGIIKPWMGGEISLRSAEK
Predicted molecular weight61 kDa including tags
Amino acids2 to 321
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab198449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.49% Glutathione
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Bifunctional protein birA
- Bio R
RelevanceBirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.
Image A: SDS-PAGE with Coomassie blue showing:
Lane 1: Unbiotinylated BirA
Lane 2: ab198449
Lane 3: BRD4-Avi-biotin control
Lane 4: Protein markers
Image B. Shows the corresponding Western blot probed with Streptavidin-HRP and developed with ECL reagent.
10% SDS-PAGE with Coomassie staining: 4 µg ab198449.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198449 has not yet been referenced specifically in any publications.