Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Escherichia coli
    • Sequence

      KDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL PEPIQLLNAK QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG RKWFSPFGAN LYLSMFWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEESVVN QGWITLQEAG INLDRNTLAA MLIRELRAAL ELFEQEGLAP YLSRWEKLDN FINRPVKLII GDKEIFGISR GIDKQGALLL EQDGIIKPWM GGEISLRSAE K
    • Molecular weight

      37 kDa including tags
    • Amino acids

      2 to 321
    • Tags

      His-DDDDK tag N-Terminus
    • Additional sequence information

      NP_418404

Specifications

Our Abpromise guarantee covers the use of ab135015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    ab135015 (10 µg/ml) completely biotinylated 10 µM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity

    >= 65 % SDS-PAGE.

  • Form

    Liquid
  • Additional notes

    Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.02% Imidazole
    Constituents: 0.05% DTT, 0.79% Tris HCl, 5% Glycerol, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Bifunctional protein birA
    • Bio R
    • BioR
    • Biotin [acetyl CoA carboxylase] synthetase
    • Biotin ligase
    • Biotin operon repressor
    • Biotin protein ligase
    • Bir A
    • BirA bifunctional protein
    • Dhb B
    • dhbB
    see all
  • Relevance

    BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.

Images

  • 10% SDS-PAGE analysis of ab135015 (4 µg) stained with Coomassie Blue.
  • MBP-Avi biotinylation with His-FLAG-BirA using the standard reaction conditions as outlined in Enzyme Activity.

    A. Coomassie blue stained gel showing
    1. unbiotinylated MBP-Avi
    2. His-FLAG-BirA / MBPAvi
    3. MBP-Avi-biotin control

    B. The corresponding Western blot probed with Streptavidin-HRP and developed with DAB (3,3’-Diaminobenzidine) substrate.

References

ab135015 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.



Ab135015 is the protein only, without any additional buffers for reaction. This product does contain a His tag.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



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