Key features and details
- Expression system: Escherichia coli
- Purity: >= 65% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant E. coli BirA protein (Active)
See all BirA proteins and peptides
Biological activityab135015 (10 µg/ml) completely biotinylated 10 µM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50 mM Bicine pH 8.3, 10 mM ATP, 10 mM MgOAc, and 50 µM d-Biotin.
Purity>= 65 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceKDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL PEPIQLLNAK QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG RKWFSPFGAN LYLSMFWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEESVVN QGWITLQEAG INLDRNTLAA MLIRELRAAL ELFEQEGLAP YLSRWEKLDN FINRPVKLII GDKEIFGISR GIDKQGALLL EQDGIIKPWM GGEISLRSAE K
Predicted molecular weight37 kDa including tags
Amino acids2 to 321
TagsHis-DDDDK tag N-Terminus
Additional sequence informationNP_418404
Our Abpromise guarantee covers the use of ab135015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.02% Imidazole
Constituents: 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 5% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Bifunctional protein birA
- Bio R
RelevanceBirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins.
10% SDS-PAGE analysis of ab135015 (4 µg) stained with Coomassie Blue.
MBP-Avi biotinylation with His-FLAG-BirA using the standard reaction conditions as outlined in Enzyme Activity.
A. Coomassie blue stained gel showing
1. unbiotinylated MBP-Avi
2. His-FLAG-BirA / MBPAvi
3. MBP-Avi-biotin control
B. The corresponding Western blot probed with Streptavidin-HRP and developed with DAB (3,3’-Diaminobenzidine) substrate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab135015 has not yet been referenced specifically in any publications.