Recombinant E. coli Carbonic anhydrase 2/CA2 protein (ab87351)

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SDS-PAGE - Recombinant <em>E. coli </em> Carbonic anhydrase 2/CA2 protein (ab87351)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant E. coli Carbonic anhydrase 2/CA2 protein
      See all Carbonic anhydrase 2/CA2 proteins and peptides

    • Purity

      > 95 % SDS-PAGE.
      ab87351 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Escherichia coli

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

    Specifications

    Our Abpromise guarantee covers the use of ab87351 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.242% Tris

    General Info

    • Alternative names

      • CA 2
      • CA II
      • CA-II
      • Ca2
      • CAC
      • CAH2_HUMAN
      • CAII
      • Car 2
      • Car2
      • Carbonate dehydratase II
      • Carbonic anhydrase 2
      • Carbonic anhydrase B
      • Carbonic anhydrase C
      • Carbonic anhydrase C, formerly
      • Carbonic anhydrase II
      • Carbonic dehydratase
      • epididymis luminal protein 76
      • Epididymis secretory protein Li 282
      • HEL-76
      • HEL-S-282
      see all

    • Function

      Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.

    • Involvement in disease

      Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.

    • Sequence similarities

      Belongs to the alpha-carbonic anhydrase family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> Carbonic anhydrase 2/CA2 protein (ab87351)
      SDS-PAGE - Recombinant E. coli Carbonic anhydrase 2/CA2 protein (ab87351)
      ab87351 on 15% SDS-PAGE (3µg)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab87351? Please let us know so that we can cite the reference in this datasheet.

    ab87351 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-8 of 8 Abreviews or Q&A

    Question

    I would prefer a refund if possible- thanks.

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    Answer

    Thanks for letting me know. I have changed this credit to a refund. You can use the same credit ID number I provided earlier.

    I hope this is helpful. Please let me know if you need anything further.

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    Question

    I am sorry- does this mean I can only use it against new
    purchases? or can you simply credit my account?

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    Answer

    In this case I have issued you a "store credit" for a future purchase at Abcam. If you like I can change it to a refund.

    Please let me know how you wish to proceed.

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    Question


    I need to wait till the next user stop using the SPR system, so I can
    get the exact info about the lack of binding to NTA chip.
    I would like to receive a credit for that purchase-

    Read More
    Answer

    Thank you for contacting us.


    I am sorry that this protein did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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    Question

    I am not sure I understand: the protein may not have a functional
    His-tag nor does it have Carbonic Anhydrase activity and this is fine?
    I am sorry but the info about lack of activity is not listed under
    product description- i.e. you are selling a protein that you know is
    inactive and you are not sharing this info in product
    description? At least there should be a note additional info under
    "Scientific Support". Sorry but this is not obvious (nor expected)
    that one can sell inactive protein and assume this is fine.

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    Answer

    Thanks for your reply.

    Upon re-reading your first email I did realize that you state the protein did not bind the NTA chip, which if it is His tagged it certainly should bind this chip. I apologize for this oversight. For this reason I am happy to offer a free replacement, credit or refund on your purchase of ab87351.You do not need to return the unused portion.

    Please let me clarify as well that I did not mean to suggest we know this protein is not active. We just have no functional data to suggest whether this protein is or is not active. Functionality has not, to our knowledge,been tested. We would describe the protein as 'active' if it had tested positive in a functional assay.

    You may wish to submit a review of your experience using this protein using our 'Abreview' customer review system. Your review will be published on the online datasheet for ab87351 so that other researchers may see your comments and results. For publishing reviews, we offer 'points' which can be used for discounts on future purchases or Amazon gift cards. For more information, please see: https://www.abcam.com/reviews.

    I hope this is helpful. I look forward to hearing from you so that we may resolve this issue for you as soon as possible.

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    Question


    product: ab87351 Carbonic anhydrase His-tagged

    I purchased this protein to serve as a positive control for SPR experiment on an NTA-chip. This protein had not been captured on the NTA surface and whatever little was captured was inactive.

    I have a positive control- CAII bovine from sigma, I know it binds the small molecules that I work with, while the His-tagged protein from your source remained completely inactive.

    I would like to return this product as this protein is dead in CA activities. please provide me with RMA and shipping instructions.


    PS. your shipping label is printed with such a tiny print (I am guessing it is size 65 or smaller) that is virtually unreadable without magnifying glass.

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    Answer

    Thank you for your enquiry.

    Thus far the only data we have regardingab87351is that the protein is the correct amino acid sequence and a protein of the expected molecular weight can be purified and stained via commassie. This Carbonic Anhydrase II recombinant protein has not previouslybeen tested for activity or any other functional properties and as such we cannot guarantee functional activity from this product.

    Also please note that a similar question regarding potential of ab87351 to have activity, asked in March 2012,is recorded under the 'Scientific Support' tab onour online product datasheet. Please see the text of the question and answer below:

    Thursday 29-March-2012




    Is this protein still active? Trying to use in enzymatic activity assay.


    ANSWER:
    Thank you for contacting Abcam regarding ab87351.


    Regrettably, our protein's biological activity hasn't been tested yet. I should mention that it hasn't been exposed to denaturing agents, so it is possible that it's bioactive, but we can not guarantee it.





    I hope this is helpful. Please contact us again if you have any further questions.

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    Question

    Dear apcam support team, I am interested in purified His-tagged streptavidin (ab78833) and carbonic anhydrase II (ab87351). Unfortunately both proteins are stored in tris-containing buffer. Would it also be possible to order both protein in PBS instead of tris? If yes, can you let me know what this would cost in addition? Thanks in advance. Regards,

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    Answer

    Thank you for contacting us.

    I am afraid these products are provided in Tris buffer to maintain their stability and it is the only form they are available in our catalogue.

    I am very sorry I could not be more helpful, and appreciate your comprehension. Please do not hesitate to contact us for further assistance.

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    Question

    Ni++ column conditions used including buffer and protocol

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    Answer

    Thank you for contacting us.

    I received the following answers from the lab to your questions:

    1) Do you have a protocol and buffer information for using this His-tagged protein on a Ni++ column?

    - We regrettably do not share our purification protocol, but the buffer used is 20 mM Tris pH8.0, 0.5M NaCl, 5mM imidazole.

    2)Assumablya Ni++ column was used to purify the protein. What conditions were specifically used?

    - No special conditions were used during the purification.


    I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Is this protein still active? Trying to use in enzymatic activity assay.

    Read More
    Answer

    Thank you for contacting Abcam regarding ab87351.


    Regrettably, our protein's biological activity hasn't been tested yet. I should mention that it hasn't been exposed to denaturing agents, so it is possible that it's bioactive, but we can not guarantee it.


    I apologize for any inconvenience this may cause. Please do not hesitate to contact us if you have any additional questions.

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