Description

  • Product name

    Recombinant E. coli fur protein
  • Purity

    > 95 % SDS-PAGE.
    ab78816 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Escherichia coli
    • Sequence

      MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI RLTNHSLYLY GHCAEGDCRE DEHAHEGK

Specifications

Our Abpromise guarantee covers the use of ab78816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0222% Calcium chloride, 0.00174% PMSF, 0.242% Tris, 0.58% Sodium chloride

General Info

  • Alternative names

    • DNA binding transcriptional dual regulator of siderophore biosynthesis and transport
    • ECK0671
    • Ferric uptake regulation protein
    • Ferric uptake regulator
    • FUR
    • JW0669
    see all
  • Relevance

    fur (ferric uptake regulator) protein is a DNA-binding protein which regulates iron-responsive genes. A small, 17-kDa, global transcriptional repressor, fur, in the presence of iron regulates functions as diverse as iron acquisition, oxidative stress, and virulence. In Escherichia coli, members of the fur family regulate the expression of more than 100 genes that function in processes as varied as the biosynthesis and transport of siderophores, the expression of virulence factors, the alleviation of oxidative and NO-induced stress, and the inhibition of ferritin production through the expression of RyhB.
  • Cellular localization

    Cytoplasmic

Images

  • 15% SDS-PAGE showing ab78816 at approximately 16kDa (3µg).

References

ab78816 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your email.

I will arrange for the replacement of the 3 vials as soon as a new lot is available.

I will be in touch and I apologize for all the trouble and inconvenience.

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Answer

Thank you for your reply.

Yes, I'd be happy to replace all those vials that did not work.

I have found one order from xxxx.

Please let me know if there were any other orders where the vials were showing degradation. If not, I will arrange the free of charge replacement for the 2 vials mentioned above.

I look forward to hear back from you and resolve this case.

Read More

Answer

Thank you for your patience.

The lab retested the 2lots and the protein is indeed degraded. We are very sorry for this inconvenience and apologize about any problems caused.

A new lot would be available in about 2-3 weeks.

I can offer you either a free of charge replacement with the new lot, a credit or refund. Please let me know which option would be best for you.

I look forward to hear back and resolve this problem for you.

Read More

Answer

Thank you for contacting us.

I have heard back from the lab with the following information:

We haven not obtained a similar pattern. We will verify whether these additional bands represent degradation by re-testing them today.

The protein has not been exposed to denaturing agents.
As soon as the lab has the result of the SDS-PAGE, I will email you.
In the meanwhile, please contact me without hesitation if you have any additional questions.

Read More

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