Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein (ab87414)

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SDS-PAGE - Recombinant <em>E. coli </em> Glucose 6 Phosphate Dehydrogenase protein (ab87414)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein
      See all Glucose 6 Phosphate Dehydrogenase proteins and peptides

    • Biological activity

      Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.

    • Purity

      > 90 % SDS-PAGE.
      Purified by conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Escherichia coli

      • Sequence

        MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

    Specifications

    Our Abpromise guarantee covers the use of ab87414 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C.

      pH: 7.4
      Constituents: 0.975% MES, 0.00174% PMSF, 0.0077% DTT, 0.0584% EDTA, 10% Glycerol (glycerin, glycerine)

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • G6PD
      • G6PD_HUMAN
      • G6PD1
      • G6pdx
      • Glucose 6 phosphate 1 dehydrogenase
      • Glucose 6 phosphate dehydrogenase
      • Glucose 6 phosphate dehydrogenase, G6PD
      • Glucose-6-phosphate 1-dehydrogenase
      • MET19
      • POS10
      • Zwf1p
      see all

    • Function

      Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

    • Tissue specificity

      Isoform Long is found in lymphoblasts, granulocytes and sperm.

    • Pathway

      Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.

    • Involvement in disease

      Anemia, non-spherocytic hemolytic, due to G6PD deficiency

    • Sequence similarities

      Belongs to the glucose-6-phosphate dehydrogenase family.

    • Post-translational
      modifications

      Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> Glucose 6 Phosphate Dehydrogenase protein (ab87414)
      SDS-PAGE - Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein (ab87414)
      ab87414 on 15% SDS-PAGE (4µg)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab87414? Please let us know so that we can cite the reference in this datasheet.

    ab87414 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Inquiry: Hi, I was wondering what the conformation of the his-tagged Glucose 6 phosphate dehydrogenase is (dimer, tetramer..) ? Thank you,

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    Answer

    Thank you for your enquiry.

    I can confirm that ab87414 is a recombinant full length E coli Glucose 6 Phosphate Dehydrogenase and it is not tagged.

    If you need any further assistance in the future, please do not hesitate to contact me.

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