Recombinant E. coli Glucose 6 Phosphate Dehydrogenase protein (ab87414)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Escherichia coli
    • Sequence
      MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Specifications

Our Abpromise guarantee covers the use of ab87414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.
    Purified by conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Glycerol, 50mM MES, 2mM EDTA, 0.5mM DTT, 0.1mM PMSF, pH 6

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • G6PD
    • G6PD_HUMAN
    • G6PD1
    • G6pdx
    • Glucose 6 phosphate 1 dehydrogenase
    • Glucose 6 phosphate dehydrogenase
    • Glucose 6 phosphate dehydrogenase, G6PD
    • Glucose-6-phosphate 1-dehydrogenase
    • MET19
    • POS10
    • Zwf1p
    see all
  • Function
    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • Tissue specificity
    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • Pathway
    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • Involvement in disease
    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • Sequence similarities
    Belongs to the glucose-6-phosphate dehydrogenase family.
  • Post-translational
    modifications
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt

Images

  • SDS-PAGE - Glucose 6 Phosphate Dehydrogenase protein (ab87414)
    SDS-PAGE - Glucose 6 Phosphate Dehydrogenase protein (ab87414)
    ab87414 on 15% SDS-PAGE (4µg)

References

ab87414 has not yet been referenced specifically in any publications.

Publishing research using ab87414? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

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Inquiry: Hi, I was wondering what the conformation of the his-tagged Glucose 6 phosphate dehydrogenase is (dimer, tetramer..) ? Thank you,

Thank you for your enquiry.

I can confirm that ab87414 is a recombinant full length E coli Glucose 6 Phosphate Dehydrogenase and it is not tagged.

If you need any further assistance in the future, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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