Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant E. coli Hsp40 protein
See all Hsp40 proteins and peptides
Biological activityProtein Refolding Assay: Positive Tested positive for its ability to stimulate DnaK's protein-refolding activity in the presence of GrpE.
Purity> 90 % SDS-PAGE.
> 90% pure as determined by SDS-PAGE and Western Blot analysis
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab91598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesDnaJ has a tendency to aggregate in solution with ionic strength lower than 0.1 M KCl. Where lower salt conditions are required, addition of 0.05% Triton-X100 or Brij 58 is recommended.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 1.5% Potassium chloride, 0.077% DTT, 0.595% HEPES, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- DnaJ (Hsp40) homolog subfmaily B member 1
- DNAJ 1
- DNAJ B1
FunctionInteracts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Sequence similaritiesContains 1 J domain.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91598 has not yet been referenced specifically in any publications.