Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, MS
Product nameRecombinant E. coli IdhA protein
See all IdhA proteins and peptides
Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Purity> 95 % SDS-PAGE.
ab208298 was purified using conventional chromatography techniques.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight39 kDa including tags
Amino acids1 to 329
TagsHis tag N-Terminus
Additional sequence informationNP_415898.
Our Abpromise guarantee covers the use of ab208298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- D-lactate dehydrogenase
- Fermentative lactate dehydrogenase
RelevanceIdhA, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab208298 has not yet been referenced specifically in any publications.