Recombinant E. coli mdh protein (ab124594)

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SDS-PAGE - Recombinant <em>E. coli </em> mdh protein (ab124594)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant E. coli mdh protein
      See all mdh proteins and peptides

    • Purity

      > 95 % SDS-PAGE.
      ab124594 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P61889

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Escherichia coli

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

      • Predicted molecular weight

        35 kDa including tags

      • Amino acids

        1 to 312

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab124594 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

    General Info

    • Alternative names

      • Malate dehydrogenase
      • mdh
      • MDH_ECOLI

    • Function

      Catalyzes the reversible oxidation of malate to oxaloacetate.

    • Sequence similarities

      Belongs to the LDH/MDH superfamily. MDH type 1 family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> mdh protein (ab124594)
      SDS-PAGE - Recombinant E. coli mdh protein (ab124594)
      15% SDS-PAGE showing ab124594 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab124594? Please let us know so that we can cite the reference in this datasheet.

    ab124594 has been referenced in 1 publication.

    • Shaulov Y  et al. Escherichia coli mediated resistance of Entamoeba histolytica to oxidative stress is triggered by oxaloacetate. PLoS Pathog 14:e1007295 (2018). PubMed: 30308066

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