Recombinant E. coli mdh protein (ab168033)

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SDS-PAGE - Recombinant <em>E. coli </em> mdh protein (ab168033)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant E. coli mdh protein
      See all mdh proteins and peptides

    • Biological activity

      Specific activity is > 450 units/mg, and is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P61889

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Escherichia coli

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

      • Predicted molecular weight

        35 kDa including tags

      • Amino acids

        1 to 312

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab168033 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.29% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Malate dehydrogenase
      • mdh
      • MDH_ECOLI

    • Function

      Catalyzes the reversible oxidation of malate to oxaloacetate.

    • Sequence similarities

      Belongs to the LDH/MDH superfamily. MDH type 1 family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> mdh protein (ab168033)
      SDS-PAGE - Recombinant E. coli mdh protein (ab168033)
      15% SDS-PAGE analysis of ab168033 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab168033? Please let us know so that we can cite the reference in this datasheet.

    ab168033 has not yet been referenced specifically in any publications.

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