Recombinant E. coli mdh protein (ab168033)
- Datasheet
- References
- Protocols
Overview
-
Product nameRecombinant E. coli mdh protein
See all mdh proteins and peptides -
Protein lengthFull length protein
Description
-
NatureRecombinant
-
SourceEscherichia coli
-
Amino Acid Sequence
-
Accession
-
SpeciesEscherichia coli
-
SequenceMGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK
-
Molecular weight35 kDa including tags
-
Amino acids1 to 312
-
TagsHis tag N-Terminus
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab168033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Biological activitySpecific activity is > 450 units/mg, and is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.
-
Applications
SDS-PAGE
Functional Studies
-
Purity>95% by SDS-PAGE .
-
FormLiquid
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.29% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- Malate dehydrogenase
- mdh
- MDH_ECOLI
-
FunctionCatalyzes the reversible oxidation of malate to oxaloacetate.
-
Sequence similaritiesBelongs to the LDH/MDH superfamily. MDH type 1 family.
- Information by UniProt
Images
-
SDS-PAGE - Recombinant E. coli mdh protein (ab168033)15% SDS-PAGE analysis of ab168033 (3µg).
Datasheets and documents
References
ab168033 has not yet been referenced specifically in any publications.
