Recombinant E. coli mdh protein (ab168033)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P61889
    • Species
      Escherichia coli
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK
    • Molecular weight
      35 kDa including tags
    • Amino acids
      1 to 312
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab168033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity is > 450 units/mg, and is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >95% by SDS-PAGE .

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Malate dehydrogenase
    • mdh
    • MDH_ECOLI
  • Function
    Catalyzes the reversible oxidation of malate to oxaloacetate.
  • Sequence similarities
    Belongs to the LDH/MDH superfamily. MDH type 1 family.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant <em>E. coli </em> mdh protein (ab168033)
    SDS-PAGE - Recombinant E. coli mdh protein (ab168033)
    15% SDS-PAGE analysis of ab168033 (3µg).

References

ab168033 has not yet been referenced specifically in any publications.

Publishing research using ab168033? Please let us know so that we can cite the reference in this datasheet.

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