Key features and details
- Expression system: Escherichia coli
- Suitable for: WB
Product nameRecombinant E. coli Multi Tag protein
Biological activityThis protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight45 kDa
Our Abpromise guarantee covers the use of ab36791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis.
This product is a bacterial lysate provided in1 x reduced SDS-PAGE loading buffer. It is effective as a positive control for epitope tag antibodies in immunoblotting.
For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 0.76% Tris, 2% Sodium lauryl sulfate, 10% Glycerol, 0.01% Bromophenol blue
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab36791 has been referenced in 1 publication.
- Trofka A et al. The Her7 node modulates the network topology of the zebrafish segmentation clock via sequestration of the Hes6 hub. Development 139:940-7 (2012). WB . PubMed: 22278920