Question (77076) | Recombinant E. coli Multi Tag protein (ab36791)

Go to datasheet (ab36791)

Question

I am planning to quantify a myc-tagged protein in a lysate by Western Blot. I am thus wondering if product ab36791 (multi-tag refernece protein) is a purified recombinant protein that comes at a specific (lot-specific) protein concentration or is it just a bacterial lysate?

Answer

I confirm that the multi-tag protein ab36791 is prepared from the whole bacteria lysate, therefore the concentration cannot be determined.
The recommended usage of this product is between 0.5 and 2 µl per well for a standard western blot procedure.

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