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I am planning to quantify a myc-tagged protein in a lysate by Western Blot. I am thus wondering if product ab36791 (multi-tag refernece protein) is a purified recombinant protein that comes at a specific (lot-specific) protein concentration or is it just a bacterial lysate?
Asked on Nov 13 2017
I confirm that the multi-tag protein ab36791 is prepared from the whole bacteria lysate, therefore the concentration cannot be determined.
The recommended usage of this product is between 0.5 and 2 µl per well for a standard western blot procedure.
Answered on Nov 13 2017