Recombinant E. coli ndk protein (ab123167)

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SDS-PAGE - Recombinant <em>E. coli </em> ndk protein (ab123167)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant E. coli ndk protein
      See all ndk proteins and peptides

    • Purity

      > 95 % SDS-PAGE.
      ab123167 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P0A763

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Escherichia coli

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSHMAIERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR

      • Predicted molecular weight

        18 kDa including tags

      • Amino acids

        1 to 143

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab123167 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • NDK
      • NDK_ECOLI
      • NDP kinase
      • Nucleoside diphosphate kinase
      • Nucleoside-2-P kinase
      see all

    • Function

      Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.

    • Sequence similarities

      Belongs to the NDK family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> ndk protein (ab123167)
      SDS-PAGE - Recombinant E. coli ndk protein (ab123167)
      15% SDS-PAGE showing ab123167 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab123167? Please let us know so that we can cite the reference in this datasheet.

    ab123167 has not yet been referenced specifically in any publications.

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