Recombinant E. coli PPIA protein (His tag) (ab225608)

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SDS-PAGE - Recombinant <em>E. coli </em> PPIA protein (His tag) (ab225608)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant E. coli PPIA protein (His tag)

    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P0AFL5

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Escherichia coli

      • Sequence

        AKGDPHVLLTTSAGNIELELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVI PGFMIQGGGFTEQMQQKKPNPPIKNEADNGLRNTRGTIAMARTADKDSAT SQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVADKISQVPTHDVGPYQ NVPSKPVVILSAKVLP

      • Predicted molecular weight

        34 kDa including tags

      • Amino acids

        25 to 190

      • Tags

        His tag N-Terminus

      • Additional sequence information

        Escherichia coli O157:H7. This product is the full-length mature protein from aa 25 to 190 with a 6xHis-SUMO tag at the N-terminus. The signal peptide is not included.

    Specifications

    Our Abpromise guarantee covers the use of ab225608 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer

    General Info

    • Alternative names

      • Cyclophilin A
      • Peptidyl-prolyl cis-trans isomerase A
      • PPIA
      • PPIA_ECO57
      • PPIase A
      • Rotamase A
      see all

    • Function

      PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

    • Sequence similarities

      Belongs to the cyclophilin-type PPIase family.
      Contains 1 PPIase cyclophilin-type domain.

    • Cellular localization

      Periplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> PPIA protein (His tag) (ab225608)
      SDS-PAGE - Recombinant E. coli PPIA protein (His tag) (ab225608)

      (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225608 with 5% enrichment gel and 15% separation gel.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab225608? Please let us know so that we can cite the reference in this datasheet.

    ab225608 has not yet been referenced specifically in any publications.

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