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Could you please tell us what is the relation between µg and unit of this product (how many unit in 1 µg of E coli DNA helicase)?
- What is the DNA helicase quantity or concentration required to unwind 1µg DNA do you recommend?
Asked on Sep 27 2013
I have contacted the lab regarding your enquiry. They have confirmed that RecQ helicase activity will depend on structures of DNA duplex.
The following paper describes details of assay conditions with various forms of DNA substrates which I think may be very helpful to you:
Hishida T et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004).
Elisa ThomasAbcam Scientific Support
Answered on Sep 27 2013