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Does this protein have an affinity tag that was used for purification? If so, what is the tag?
Asked on Nov 24 2015
The RecQ helicase is full-length recombinant protein without any tag, expressed in E.coli. The earliest reference on the datasheet describes production of the protein.
Hishida T et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004).
Tom RuyleAbcam Scientific Support
Answered on Nov 24 2015