The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab113149 was purified using conventional chromatography techniques.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00 Constituents: 0.32% Tris HCl, 10% Glycerol, 0.04% DTT
RNase H from E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA in RNA:DNA duplexes to generate products with 3'-hydroxyl and 5'-phosphate ends.1,2,3 RNase H degrades only the RNA component of the DNA-RNA hybrid (RNA that is hydrogen bonded to a complementary DNA strand). Other enzymes in E. coli which degrade RNA in the DNA-RNA hybrid are DNA polymerase I and exonuclease III, but these degrade either the RNA or DNA of the hybrids. Ribonuclease H will not cleave single-stranded or double-stranded DNA or RNA.1,2
SDS-PAGE - RNase H protein (ab113149)
15% SDS-PAGE showing ab113149 (3 µg) at approximately 20 kDa.
has not yet been referenced specifically in any publications.
Publishing research using ab113149? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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