Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant E. coli RNase H protein
Biological activitySpecific Activity: 100,000 units/mg protein. Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37°C. Endo- and exo-DNase activities and RNase activity were not detected with 100 U/ml RNaseH in 50 ul reaction at 37°C.
Purity> 95 % SDS-PAGE.
Greater than 95% protein determined by SDS-PAGE (CBB staining) Endo- and exo-DNase activities and RNase activity were not detected with 100 U/ml RNaseH in 50 ul reaction at 37 degrees C.
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab91360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
To avoid contamination of trace amounts of nucleic acids in BSA, use reaction buffer that does not contain BSA and use RNaseH at higher concentrations.
1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H-labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37°C.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 50% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Ribonuclease H
- Ribonuclease HI
- RNase H
RelevanceRNase H from E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA in RNA:DNA duplexes to generate products with 3'-hydroxyl and 5'-phosphate ends.1,2,3 RNase H degrades only the RNA component of the DNA-RNA hybrid (RNA that is hydrogen bonded to a complementary DNA strand). Other enzymes in E. coli which degrade RNA in the DNA-RNA hybrid are DNA polymerase I and exonuclease III, but these degrade either the RNA or DNA of the hybrids. Ribonuclease H will not cleave single-stranded or double-stranded DNA or RNA.1,2
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91360 has not yet been referenced specifically in any publications.