Product nameRecombinant E. coli RuvC protein (Active)
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDD LPSRLKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIV AAVNQELPVFEYAARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADA ADALAIAITHCHVSQNAMQMSESRLNLARGRLR
Our Abpromise guarantee covers the use of ab63828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab63828 can be used for: 1) Studies on the homologous recombination mechanism. 2) To use as an endonuclease which functions specifically to the Holliday structure.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Stable for 12 months at -20°C.
Constituents: 0.039% Beta mercaptoethanol, 0.158% Tris HCl, 0.0584% EDTA, 50% Glycerol, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Crossover junction endodeoxyribonuclease ruvC
- Holliday junction nuclease ruvC
- Holliday junction resolvase ruvC
RelevanceIn Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double stranded DNA and enhances ATPase activity of RuvB. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab63828 has been referenced in 2 publications.
- Nicholls TJ et al. Topoisomerase 3a Is Required for Decatenation and Segregation of Human mtDNA. Mol Cell 69:9-23.e6 (2018). PubMed: 29290614
- García-Luis J & Machín F Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions. Nat Commun 5:5652 (2014). PubMed: 25466415