Recombinant E. coli Thioredoxin 1 protein (ab167980)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant E. coli Thioredoxin 1 protein -
Biological activity
Specific activity is 16-18 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. -
Purity
> 85 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MHHHHHHMGSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPI LDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKV GALSKGQLKEFLDANLAGS -
Predicted molecular weight
13 kDa including tags -
Amino acids
2 to 109 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab167980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 10% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- ADF
- ATL-derived factor
- SASP
see all -
Relevance
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/ dithiol group within the conserved Cys-Gly-Pro-Cys active site. Specific protein disulfide targets for reduction by Trx include protein disulfide –isomerase (PDI) and a number of transcription factors such as p53, NFkB and AP-1. Trx is also apable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. -
Cellular localization
Cytoplasmic
Images
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SDS-PAGE - Recombinant E. coli Thioredoxin 1 protein (ab167980)15% SDS PAGE analysis of ab167980 at 3 µg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab167980 has not yet been referenced specifically in any publications.
