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    recombinant-e-coli-thioredoxin-1-protein-ab167980.pdf

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Signal Transduction Protein Trafficking ER Proteins
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Recombinant E. coli Thioredoxin 1 protein (ab167980)

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SDS-PAGE - Recombinant <em>E. coli </em> Thioredoxin 1 protein (ab167980)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    Description

    • Product name

      Recombinant E. coli Thioredoxin 1 protein
    • Biological activity

      Specific activity is 16-18 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
    • Purity

      > 85 % SDS-PAGE.

    • Expression system

      Escherichia coli
    • Accession

      P0A9P4
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Escherichia coli
      • Sequence

        MHHHHHHMGSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPI LDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKV GALSKGQLKEFLDANLAGS
      • Predicted molecular weight

        13 kDa including tags
      • Amino acids

        2 to 109
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab167980 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • ADF
      • ATL-derived factor
      • SASP
      • Surface associated sulphydryl protein
      • TRN
      • TXN
      • TXN 1
      see all
    • Relevance

      Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/ dithiol group within the conserved Cys-Gly-Pro-Cys active site. Specific protein disulfide targets for reduction by Trx include protein disulfide –isomerase (PDI) and a number of transcription factors such as p53, NFkB and AP-1. Trx is also apable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
    • Cellular localization

      Cytoplasmic

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> Thioredoxin 1 protein (ab167980)
      SDS-PAGE - Recombinant E. coli Thioredoxin 1 protein (ab167980)
      15% SDS PAGE analysis of ab167980 at 3 µg.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab167980? Please let us know so that we can cite the reference in this datasheet.

    ab167980 has not yet been referenced specifically in any publications.

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