Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Escherichia coli
    • Sequence

      MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K
    • Molecular weight

      35 kDa
    • Amino acids

      1 to 321
    • Additional sequence information

      TRXR E.coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 34.6 kDa.

Specifications

Our Abpromise guarantee covers the use of ab117175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity is 4-5 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity

    > 90 % SDS-PAGE.
    ab117175 is purified by standard chromatography.
  • Form

    Liquid
  • Additional notes

    ab117175 although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.02% DTT, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ECK0879
    • JW0871
    • Thioredoxin reductase, FAD/NAD(P)-binding
    • TRXR
  • Relevance

    Thioredoxin reductase is a member of the flavin-containing NADPH:disulfide oxidoreductases (DSOR) family.
  • Cellular localization

    Cytoplasmic

References

ab117175 has not yet been referenced specifically in any publications.

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