Recombinant E. coli UNG protein (ab171506)

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SDS-PAGE - Recombinant <em>E. coli </em> UNG protein (ab171506)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant E. coli UNG protein

    • Purity

      > 90 % SDS-PAGE.
      ab171506 is purified using conventional chromatography.

    • Expression system

      Escherichia coli

    • Accession

      P12295

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Escherichia coli

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE

      • Predicted molecular weight

        28 kDa including tags

      • Amino acids

        1 to 229

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab171506 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • DGU
      • DKFZp781L1143
      • HIGM 4
      • HIGM4
      • OTTHUMP00000240527
      • OTTHUMP00000240528
      • OTTHUMP00000240529
      • UDG
      • ung
      • UNG 1
      • UNG 15
      • UNG_HUMAN
      • UNG1
      • UNG15
      • UNG2
      • Uracil DNA glycosylase
      • Uracil DNA glycosylase 1
      • Uracil DNA glycosylase 2
      • Uracil-DNA glycosylase
      see all

    • Function

      Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

    • Tissue specificity

      Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.

    • Involvement in disease

      Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.

    • Sequence similarities

      Belongs to the uracil-DNA glycosylase family.

    • Post-translational
      modifications

      Isoform 1 is processed by cleavage of a transit peptide.

    • Cellular localization

      Mitochondrion and Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant <em>E. coli </em> UNG protein (ab171506)
      SDS-PAGE - Recombinant E. coli UNG protein (ab171506)
      15% SDS-PAGE analysis of ab171506 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab171506? Please let us know so that we can cite the reference in this datasheet.

    ab171506 has not yet been referenced specifically in any publications.

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