Recombinant E. coli UNG protein (ab171506)

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Description

  • Product name

    Recombinant E. coli UNG protein

  • Purity

    > 90 % SDS-PAGE.
    ab171506 is purified using conventional chromatography.

  • Expression system

    Escherichia coli

  • Accession

    P12295

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Escherichia coli

    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE

    • Predicted molecular weight

      28 kDa including tags

    • Amino acids

      1 to 229

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab171506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol

General Info

  • Alternative names

    • DGU
    • DKFZp781L1143
    • HIGM 4
    • HIGM4
    • OTTHUMP00000240527
    • OTTHUMP00000240528
    • OTTHUMP00000240529
    • UDG
    • ung
    • UNG 1
    • UNG 15
    • UNG_HUMAN
    • UNG1
    • UNG15
    • UNG2
    • Uracil DNA glycosylase
    • Uracil DNA glycosylase 1
    • Uracil DNA glycosylase 2
    • Uracil-DNA glycosylase
    see all

  • Function

    Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

  • Tissue specificity

    Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.

  • Involvement in disease

    Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.

  • Sequence similarities

    Belongs to the uracil-DNA glycosylase family.

  • Post-translational
    modifications

    Isoform 1 is processed by cleavage of a transit peptide.

  • Cellular localization

    Mitochondrion and Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant <em>E. coli </em> UNG protein (ab171506)
    SDS-PAGE - Recombinant E. coli UNG protein (ab171506)
    15% SDS-PAGE analysis of ab171506 (3µg).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab171506? Please let us know so that we can cite the reference in this datasheet.

ab171506 has not yet been referenced specifically in any publications.

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