Product nameRecombinant E. coli UNG protein kit
E. coli UNG protein kit (ab215390) catalyzes the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 fewer bases). It is active over a broad pH range with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200 mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. This product can be used for: Glycosylase mediated single nucleotide polymorphism detection (GMPD). Site-directed mutagenesis. As a probe for protein-DNA interaction studies. Rapid and efficient cloning of PCR products. Elimination carry-over contamination in PCR. Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C.
1 Unit is defined as the amount of UDG enzyme that catalyzes release of 60 pmol of Uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50 µL in 30 minutes at 37°C in 1X UNG Reaction Buffer (200 mM Tris-HCl (pH 8.0 at 25°C), 10 mM DTT and 10 mM EDTA) with 1 Unit of UNG and 0.2 µg [3H]-Uracil DNA (104- 105 cpm/µg).
The specific activity is 5 U/µL.
The UNG protein was purified by standard chromatographic techniques.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 2000 units 10X UNG Reaction Buffer 1 x 2ml UNG Enzyme 1 vial UNG Storage Buffer 1 x 2ml
FunctionExcises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Tissue specificityIsoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
Involvement in diseaseDefects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
Sequence similaritiesBelongs to the uracil-DNA glycosylase family.
modificationsIsoform 1 is processed by cleavage of a transit peptide.
Cellular localizationMitochondrion and Nucleus.
- Information by UniProt
- HIGM 4
ab215390 has not yet been referenced specifically in any publications.