Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255)

Description

  • Product name

    Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag)
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Predicted molecular weight

      50 kDa including tags

Specifications

Our Abpromise guarantee covers the use of ab230255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    ELISA

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituent: PBS

    (supplemented with glycerol, arginine and glutamic acid).

Images

  • SDS-PAGE analysis of 1 µg and 5 µg (lanes 1-2) of ab230255 under denaturing and reducing conditions.

    Two prominent bands: E3E2 at ~50 kDa and cleaved E2 at ~40 kDa.

  • All lanes : anti-EEEV E2 rabbit polyclonal antibody at 0.5 µg/ml

    Lane 1 : Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.05 µg
    Lane 2 : Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.1 µg
    Lane 3 : Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.2 µg

    Secondary
    All lanes : anti-rabbit IgG HRP conjugate


    Followed by TMB substrate. 

  • Plate was coated with ab230255 starting at 800 ng/well, serially diluted in DPBS.  Washed plate was detected using Rabbit Anti-EEEV E2 pAb at 1 µg/mL. OD650 is reported.

References

ab230255 has not yet been referenced specifically in any publications.

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