Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
Product nameRecombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag)
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight50 kDa including tags
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab230255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
(supplemented with glycerol, arginine and glutamic acid).
SDS-PAGE analysis of 1 µg and 5 µg (lanes 1-2) of ab230255 under denaturing and reducing conditions.
Two prominent bands: E3E2 at ~50 kDa and cleaved E2 at ~40 kDa.
All lanes : anti-EEEV E2 rabbit polyclonal antibody at 0.5 µg/ml
Lane 1 :
Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.05 µg
Lane 2 :
Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.1 µg
Lane 3 :
Recombinant Eastern Equine Encephalitis Virus E3E2 protein (His tag) (ab230255) at 0.2 µg
All lanes : anti-rabbit IgG HRP conjugate
Followed by TMB substrate.
Plate was coated with ab230255 starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using Rabbit Anti-EEEV E2 pAb at 1 µg/mL. OD650 is reported.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230255 has not yet been referenced specifically in any publications.