Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant EN-TEV Protease (mutated S219N) protein (Tagged-His Tag)
See all EN-TEV Protease proteins and peptides
Biological activityComplete digestion of 50µg of T7 Tag-HBxAg protein using 1µg TEV enzyme (in 1/50 ratio) in either 3 hours at 37 °C or 12 hours at 4 °C.
The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT. The duration of the cleavage reaction is typically overnight, although lots of cleavage will happen in the first few hours and prolonged incubation times may not lead to proportional increases in cleavage. TEV protease is maximally active at 34 °C, but we recommend performing the digest at room temperature (20 °C) or 4 °C. TEV protease is only three-fold less active at 4 °C than at 20 °C.
Typically, a good rule of thumb for initial test of digestion is 1 µg TEV enzyme per 50 - 100µg target protein ratio. Perform a small-scale reaction first, if possible, to gauge the efficiency of processing.
Purity> 98 % SDS-PAGE.
ab134877 is sterile-filtered.
Expression systemEscherichia coli
Protein lengthFull length protein
SpeciesTobacco etch virus
SequenceGHHHHHHHGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFII TNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFP PFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKH WIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTN QEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNRRRRR
Amino acids2037 to 2073
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab134877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 0.02% DTT, 0.79% Tris HCl, 0.015% EDTA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134877 has not yet been referenced specifically in any publications.