Recombinant EN-TEV Protease protein (TurboTEV Protease) (ab285997)
Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE
Description
-
Product name
Recombinant EN-TEV Protease protein (TurboTEV Protease)
See all EN-TEV Protease proteins and peptides -
Biological activity
≥10,000 units/mg
One unit cleaves >85% of 3 µg control substrate, Glu-Asn-Leu-Tyr-Phe-Gln-Gly, in 1 hour at 30°C.
-
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Actual molecular weight
52 kDa -
Tags
His tag N-Terminus , GST tag N-Terminus
-
-
Description
Recombinant EN-TEV Protease protein
Specifications
Our Abpromise guarantee covers the use of ab285997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 9205 TurboTEV Protease, Recombinant. 9205-1 is the same size as the 1 mg size of ab285997.
ab285997 is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly; the optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser. It contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), TurboTEV.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -20°C.
pH: 8.0
Constituents: 0.39% Tris HCl, 0.29% Sodium chloride, 0.03% TCEP, 50% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with ab285997 or TEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 14 mM β-mercaptoethanol at 4°C for 16 hours. The cleaved products are 27 kDa and 22 kDa. TEV is a competitor TEV product.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab285997 has not yet been referenced specifically in any publications.