Recombinant Envelope Glycoprotein (His tag) (ab230280)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: WB, SDS-PAGE, ELISA
Description
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Product name
Recombinant Envelope Glycoprotein (His tag)
See all Envelope Glycoprotein proteins and peptides -
Purity
> 70 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Ebolavirus -
Amino acids
33 to 676 -
Tags
His tag C-Terminus -
Additional sequence information
Minus the transmembrane domain.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab230280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
ELISA
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: PBS
Supplemented with glycerol, arginine and glutamic acid
General Info
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Alternative names
- GP
- GP 1,2
- GP1
see all -
Cellular localization
Virion membrane; Peripheral membrane protein.
Images
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SDS-PAGE analysis of 5 µg and 1 µg (lane 1, 2 respectively) ab230280.
Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
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All lanes : Rabbit anti-EBOV Envelope Glycoprotein polyclonal antibody
Lane 1 :Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.05 µg
Lane 2 :Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.1 µg
Lane 3 :Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.5 µg
Secondary
All lanes : Anti-rabbit IgG-HRP conjugateFollowed by TMB membrane substrate.
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ELISA analysis of ab230280. Plate was coated with ab230280 starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TMB substrate. OD650 is reported. Background of ab230280 coated plate positive control serum was 0.071 OD650.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab230280 has not yet been referenced specifically in any publications.