Recombinant Envelope Glycoprotein (His tag) (ab230280)

Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Ebolavirus
    • Amino acids

      33 to 676
    • Additional sequence information

      Minus the transmembrane domain.

Specifications

Our Abpromise guarantee covers the use of ab230280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

General Info

  • Alternative names

    • GP
    • GP 1,2
    • GP1
    • GP2
    • GP2-delta
    • Spike glycoprotein
    • Virion spike glycoprotein
    see all
  • Cellular localization

    Virion membrane; Peripheral membrane protein.

Images

  • SDS-PAGE analysis of 5 µg and 1 µg (lane 1, 2 respectively) ab230280.

    Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.

  • All lanes : Rabbit anti-EBOV Envelope Glycoprotein polyclonal antibody

    Lane 1 : Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.05 µg
    Lane 2 : Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.1 µg
    Lane 3 : Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.5 µg

    Secondary
    All lanes : Anti-rabbit IgG-HRP conjugate


    Followed by TMB membrane substrate.

  • ELISA analysis of ab230280. Plate was coated with ab230280 starting at 800 ng/well, serially diluted in DPBS.  Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TMB substrate.  OD650 is reported. Background of ab230280 coated plate positive control serum was 0.071 OD650

References

ab230280 has not yet been referenced specifically in any publications.

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