Recombinant Flagellin protein (ab201366)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Flagellin protein
    See all Flagellin proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    >95% by HPLC analysis.

  • Expression system

    Escherichia coli

  • Accession

    P06179

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Salmonella enterica

    • Sequence

      MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLRLEHHH HHH

    • Predicted molecular weight

      53 kDa including tags

    • Tags

      His tag C-Terminus

    • Additional sequence information

      Salmonella typhimurium Flagellin. Single non-glycosylated polypeptide chain containing 503 amino acids, with Leu, Glu and 6 × His at C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab201366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

    Lyophilized from a 0.2µm filtered solution.

  • Reconstitution
    Briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20°C to -70°C. Further dilutions should be made in appropriate buffered solutions.

General Info

  • Alternative names

    • 41 kDa antigen
    • b1923
    • flaF
    • Flagellar filament 41 kDa core protein
    • Flagellin
    • fliC
    • hag
    • JW1908
    • P41
    see all

  • Relevance

    Flagellin (FliC) is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers are polymerized to form the tail filament. FliC monomers can function as pathogen-associated molecular patterns (PAMPs), and can be detected by host cells through surface-localized toll-like receptor 5 (TLR5) and cytosolic Nod-like receptors (NLRs).

  • Cellular localization

    Secreted. Bacterial flagellum.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab201366? Please let us know so that we can cite the reference in this datasheet.

ab201366 has been referenced in 1 publication.

  • Mlcochova P  et al. TLR4-Mediated Pathway Triggers Interferon-Independent G0 Arrest and Antiviral SAMHD1 Activity in Macrophages. Cell Rep 30:3972-3980.e5 (2020). PubMed: 32209460

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