Key features and details
- Expression system: Mammalian
- Active: Yes
- Suitable for: WB
Product nameRecombinant Furin protein
See all Furin proteins and peptides
Protein lengthFull length protein
Predicted molecular weight87 kDa
Amino acids1 to 713
Our Abpromise guarantee covers the use of ab6032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesThis 81 kDa recombinant protein was obtained from cytoplasmic extracts by centrifugation and lysis of BSC-40 cells infected with recombinant vaccinia virus (VV:hFUR713t). The preparation was subjected to UV irradiation for 5 minutes to inactivate any residual virus. Activity of 1 U/µl, where 1 U (a unit of activity) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in 1 min.
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Dibasic processing enzyme
- Dibasic-processing enzyme
- FES upstream region
FunctionFurin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
Tissue specificitySeems to be expressed ubiquitously.
Sequence similaritiesBelongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain.
DomainContains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
modificationsThe inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6032 has not yet been referenced specifically in any publications.