Recombinant Goat TNF alpha protein (ab209145)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Goat TNF alpha protein
See all TNF alpha proteins and peptides -
Purity
> 95 % SDS-PAGE.
Ion-exchange chromatography. -
Expression system
Yeast -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Goat -
Sequence
LRSSSQASSN KPVAHVVANI SAPGQLRWGD SYANALKANG VELKDNQLVV PTDGLYLIYS QVLFRGHGCP STPLFLTHTI SRIAVSYQTK VNILSAIKSP CHRETPEGAE AKPWYEPIYQ GGVFQLEKGD RLSAEINQPE YLDYAESGQV YFGIIAL -
Predicted molecular weight
17 kDa -
Amino acids
78 to 234 -
Additional sequence information
Soluble form.
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Specifications
Our Abpromise guarantee covers the use of ab209145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilised -
Additional notes
ab209145 was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 90% PBS, 10% Trehalose
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ReconstitutionReconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. Once reconstituted store at – 20 °C in working aliquots and avoid repeated freeze/thaw cycles.
General Info
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Alternative names
- APC1
- APC1 protein
- Cachectin
see all -
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
Datasheets and documents
References
ab209145 has not yet been referenced specifically in any publications.
